Identification of the phylotypes involved in cis-dichloroethene and 1,4-dioxane biodegradation in soil microcosms

Co-contamination with chlorinated compounds and 1,4-dioxane has been reported at many sites. Recently, there an increased interest in bioremediation because of the potential to degrade multiple contaminants concurrently. Towards improving efficacy, current study examined laboratory microcosms (inoculated separately two soils) determine phylotypes functional genes associated biodegradation common co-contaminants (cis-dichloroethene [cDCE] 1,4-dioxane). The impact amending lactate on cDCE was also investigated. presence either or did not one soils. Lactate appeared improve initiation biological removal inoculated soil. Stable isotope probing (SIP) then used which were actively involved carbon uptake from both soil communities. most enriched for 13C assimilation included Rhodopseudomonas Rhodanobacter. Propane monooxygenase predicted (by PICRUSt2) be dominant amended microbial communities propane gene abundance values correlated other (but less abundant) 13C-1,4-dioxane assimilation. Bacteriovorax, Pseudomonas Sphingomonas. In microcosms, PICRUSt2 DNA encoding glutathione S-transferase (a known upregulated enzyme). Overall, work demonstrated concurrent by indigenous enhancement lactate. data generated responsible (as determined SIP) could incorporated into diagnostic molecular methods site characterization. results suggest should considered solvent remediation.